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. 2018 Jan 1;11(1):dmm031146. doi: 10.1242/dmm.031146

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Overexpression of individual signaling pathway components does not phenocopy the cell-spreading phenotype induced by elevated Hipk. (A) A third instar wing imaginal disc with HA-hipk^3M+GFP expressed along the dpp domain serves as the baseline phenotype/control disc. Individual pathway activators were expressed using dpp-Gal4, UAS-GFP, namely: (B) UAS-Arm^S10, (C) UAS-Stat92E, (D) UAS-Ras^act, (E) UAS-eiger, (F) constitutively active UAS-yki^S168A, (G) UAS-N^act and (H) UAS-Ci. Discs were stained for F-actin (magenta) to reveal tissue morphology and for GFP (green, white) to mark cells in which transgenes were ectopically expressed using dpp-Gal4. Scale bars: 50 μm. All crosses were done at 29°C.