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. 2018 Jan 1;11(1):dmm031716. doi: 10.1242/dmm.031716

Fig. 2.

Fig. 2.

GUMC220 and GUMC221 MEC primary cells demonstrated xenograft growth in vivo and 3D growth in vitro. (A) Representative images of xenograft histology: H&E (left), IHC for human mitochondrial protein (clone 113-1) (right). Images were taken at 40× magnification. Scale bars: 10 μm. Bottom left insets show magnified images; 220 xenografts, n=3; 221 xenografts, n=2. (B) Representative phase-contrast images from in vitro 3D Matrigel culture: single cell suspensions at day 0 (left), spheroid formation at day 7 (center), and representative H&E images of spheres (right). Images were taken at 10× magnification. Scale bars: 400 μm. Bottom left insets in day 0 and day 1 panels show magnified images. n=3. (C) Representative green fluorescence images from in vitro 3D low-adherent plate culture: single cell suspensions in CM+Y and EpiC at day 0 (left), spheroid formation in CM+Y and EpiC at day 2 (right). Images were taken at 10× magnification. Scale bars: 300 μm. Bottom left panels show magnified images. Each experiment included three cell/condition technical replicates and each experiment was completed three times.