Fig. 12.

Experimental FOV and resolution measurements for MVPLAPO 2XC. (a) Normalized fluorescence measured with high-throughput relay shown in Figs. 6 and 7 and the MVPLAPO 2XC with rear aperture underfilled to an effective NA of 0.22. Result is for 10-mm input beam diameter. (b) Same as (a), but for MVPLAPO 2XC with rear aperture underfilled to an effective NA of 0.11. (c) Lateral and axial cross section of PSF measured experimentally by imaging $0.5\text{-}\mu \mathrm{m}\text{-}\text{diameter}$ fluorescent beads embedded in agarose. Results are for high-throughput relay and MVPLAPO 2XC with rear aperture underfilled to an effective NA of 0.22. Beads were imaged both on and off axis as specified underneath cross sections (d) Same as (c), but with an MVPLAPO 2XC with rear aperture underfilled to an effective NA of 0.11. (e) Estimation of lateral resolution measured as FWHM of PSF shown in (c)–(d). (f) Same as (e), but for axial resolution.