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. 2018 Feb 19;5(2):025001. doi: 10.1117/1.NPh.5.2.025001

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Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Fig. 2 — Selecting objective lenses for LF-TPM. (a) Numerical aperture plotted against objective focal length for 45 commercially available Olympus objectives. Red curve is the best fit for the NA’s inverse relation to the focal length of the objective. (b) The FOV diameter plotted as a function of the resolution of the objective lens. Two objectives are candidates for LF-TPM (XLFLUOR4X and MVPLAPO 2XC). Red curve is the best fit for the FOV’s linear relation to the lateral resolution. (c) Diagram of circular FOV (gray) and intensity PSF squared (pink). The rectangular FOV and pixels required for sufficient sampling are also shown. (d) The space-bandwidth product of the 45 objective lenses.