Fig. 9.

Cerebral vasculature and microglia imaged over the mouse cortex with LF-TPM. (a) Maximum projection image of cerebral vessels imaged with LF-TPM after tail vein injection of fluorescein-dextran. Dimensions of the box are $8\times 8{\mathrm{mm}}^2$. (b) $1\times 1{\mathrm{mm}}^2$ FOV imaged 3-mm off axis at orange box shown in (a). (c) $1\times 1{\mathrm{mm}}^2$ FOV imaged 3-mm off axis at blue box in (a). (d) $500\times 500{\mu \mathrm{m}}^2$ FOV highlighted by dashed blue box in (c). (e) $1\times 1{\mathrm{mm}}^2$ FOV imaged 3-mm off axis at green box shown in (a). (f) $1\times 1{\mathrm{mm}}^2$ FOV imaged 3-mm off axis at red box shown in (a). (g) $500\times 500{\mu \mathrm{m}}^2$ FOV highlighted by dashed red box in (f). (h)–(n) Same as (a)–(g), but for microglia imaged in $\mathrm{Cx}3\mathrm{Cr}{1}^{\mathrm{GFP}+/-}\mathrm{mice}$.