Table 1.
No. | Primer name | Primer sequence | Annealing temperature |
---|---|---|---|
1. | DmTim0 F | GCTCATCGCTTTTCATATGTT | 57 |
2. | DmTim R | AGGATGTGATTGGTAACCAC | 57 |
3. | DmPer0 F | TACCACCACGAGGACCTCTC | 57 |
4. | DmPer R | GATGGTGTCCGACGACAAAT | 57 |
5. | Pseudoobscura per F | ACCACCACGATGACCTCCCC | 59 |
6. | Pseudoobscura per R | TTGTTCTGCAACTCCTCCGCG | 59 |
7. | Pseudoobscura tim F | ACATACCGGAAACGCACGGG | 59 |
8. | Pseudoobscura tim R | CTTGTAGATCAGCGCGATCAAC | 59 |