Table 2.

Results of the activity analysis for the hemizygous transgenic and control flies used in this study under 25 °C and 18 °C. Chi-square test was performed to compare the rhythmicity of the flies under two temperature conditions. (N = total number of flies, Narr = no. of arrhythmic flies, Nr = no. of rhythmic flies, %r = percentage of rhythmic flies, D. melanogaster Canton-S, I26 × tim19 and I26 × tim21 show more rhythmicity on 25 °C while D. pseudoobscura and I26 lines showed preference for 18 °C.

	25 °C						18 °C
Genotypes	N	Narr	Nr	%r	Period	SEM	N	Narr	Nr	%r	Period	SEM	Chi sq.	Pref	Period
D. melanogaster Canton-S	25	1	24	96	23.95	0.13	22	11	11	50.00	23.64	0.08	*	25 °C
D. pseudoobscura Flagstaff	30	20	10	33	23.70	0.16	31	4	27	87.00	22.67	0.05	*	18 °C	*
w/Y; tim01timmel/tim01 (t28s)	23	0	23	100	23.20	0.16	28	6	22	80.00	23.00	0.16
per01/Y, w; permel/+ (2A)	30	0	30	100	24.87	0.06	11	0	11	100.00	24.01	0.07		25 °C
per01/Y; tim+; perps/+ (I26)	28	9	19	67.85	31.23	0.46	30	1	29	96.60	26.77	0.07	*	18 °C	*
w/Y; tim01, timps/tim01 (tim19)	30	14	16	53.3	20.82	0.39	35	9	26	74.20	21.09	0.21		18 °C
per01/Y; tim01, timps/tim01; perps/+ (19 × 126)	46	19	27	58.69	24.51	0.79	32	28	4	12.95	24.89	0.23	*	25 °C
w/Y; tim01, timps/tim01 (tim21)	34	9	25	73.5	20.09	0.83	55	22	33	63.93	21.10	0.15		18 °C
per01/Y; tim01, timps/tim01; perps/+ (21 × 126)	41	19	22	53.65	26.54	0.63	63	43	20	34.60	24.77	0.15	*	25 °C
per01/Y; tim01, timps/tim01; perps/+ (I26 × 35)	27	21	6	22.22	23.99	0.18	26	17	9	34.60	25.16	0.43
w/Y; tim01, timps/tim01 (tim35)	32	12	20	61.2	22.32	0.15
per01/Y; +; perps/perps (I26)	26	6	20	76.92	29.60	0.48	34	4	30	88.20	26.08	0.09			*
w/Y; tim01, timps/tim01, timps (19)	28	17	11	39.2	26.16	0.23	31	19	12	38.70	24.39	0.38		18 °C
per01/Y; timpstim01/timpstim01; perps/perps (19 × 126)	47	46	1		0.00		21	19	2	9.50	22.68	0.17	na
w/Y; tim01, timps/tim01 (21)	53	30	23	4.35	25.79	0.50	29	17	12	41.30	22.75	0.13		18 °C
per01/Y; timpstimo1/timpstim01; perps/perps (21 × 126)	31	26	5	16.1	21.00	0.72	29	17	12	11.40	22.39	0.12