Figure 5.

CBS exists in oxidized and reduced states in HEK293 cells under oxidative and reductive conditions. HEK293 cells were exposed to 0.5 mm DTT or 0.5 mm oxidized GSSG-EE for 10 min followed by washing three times with ice-cold PBS buffer. Then the cells were treated with 15% (v/v) trichloroacetic acid at 4 °C for 30 min before being alkylated with 10 mm MM(PEG)₂₄. The samples were resolved on SDS-PAGE and immunoblotted (IB) for CBS. The molecular size markers in kDa are indicated at the left.