Figure 4.

Electrophysiological characterization of allosteric fragment CU2017 on human α7 nAChRs expressed in Xenopus oocytes. A, agonist-evoked responses of human α7 nAChRs using 200 μm acetylcholine, first applied alone and then co-applied with increasing concentrations of CU2017 (5–200 μm). B, plot of the peak current responses as a function of fragment concentration and a curve fit of the data with the Hill equation. Data are shown as a scatter plot (red circles) and the mean ± S.D. of 16 independent experiments. The Hill plot has an IC₅₀ value of 26.7 ± 1.6 μm and a coefficient of 1.3 ± 0.1 (n = 16).