Figure 10.
Heme binding of HemW (RSAD1) from humans. A, for heme staining, purified RSAD1/HemW proteins of H. sapiens (lanes 1 and 3) and E. coli (lanes 2 and 4) were incubated with heme overnight and separated via SDS-PAGE. The proteins were stained with InstantBlueTM (Expedeon Inc., San Diego, CA) (lanes 1 and 2) or blotted onto nitrocellulose membrane with subsequent ECL treatment for heme staining (lanes 3 and 4). A marker indicates the relative molecular mass of the separated proteins. B, UV-visible spectra of H. sapiens RSAD1 incubated with heme overnight. An absorption maximum at 410 nm indicated heme binding. Peaks at 531 and 556 nm are typical for incorporated heme.