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. 2017 Dec 5;293(7):2330–2341. doi: 10.1074/jbc.RA117.000121

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Figure 4. — PvdO catalyzes the oxidation of dihydropyoverdine. A, analysis of pyoverdines produced by wildtype P. fluorescens A506 (top) and the corresponding dihydropyoverdines (DH-PVD) produced by the pvdO deletion strain (bottom). Chromatograms on the left depict the elution profiles of the numbered compounds that are assigned in B and D. C, detection of fluorescence (left) or iron-binding capacity in a CAS overlay assay (right) after isoelectric focusing of the pyoverdines of indicated strains. WT, wildtype A506; ΔpvdO, strains with pvdO deletion; ΔpvdO/ΔpvdN, strain with an additional pvdN deletion; ΔpvdO/pvdO, pvdO-complemented pvdO deletion strain.