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Analysis of physiological functionality of PvdO variants with single-amino acid exchanges. A and C, CAA plates containing iron-depleting EDDHA; B and D, CAA plates without EDDHA. A and B show the growth of spotted cultures on the respective media, whereas B and D show the fluorescence caused by produced pyoverdines. The amino acid exchanges and control strains are denoted in B. All plates contain the same organization of spotted strains.
