Table 1.
Primers used in this study
| Name | Sequence | Restriction site | Purpose |
|---|---|---|---|
| pEXH5-RBS-F-MR | GGCGCGGGATCCGTTTAACTTTAAGAAGGAGATATAC | BamHI | Forward primer for subcloning from pEXH5 into pME6010 |
| pEXH5-strep-term-R-MR | CCCCTTGAATTCAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTTTACTTTTCGAACTGCGGGTGGCTCC | EcoRI | Reverse primer for subcloning from pEXH5 into pME6010 |
| pEXPT7-HA-term-R-MR | CCCCTTGAATTCAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTTTAGGCGTAGTCCGGCACGTCGTACGGG | EcoRI | Reverse primer for subcloning from pEXH5 into pME6010 with HA-tag |
| PfA506-dpvdNO-F2-MR | CTAACCACCACGAAGTAAGTCCATGACCCGCGAACTCTAATATCTTGTAGG | Forward primer for pvdMNO right flanking region | |
| PfA506-matPvdO-strep-R-MR | CCGATTAAGCTTTTACTTTTCGAACTGCGGGTGGCTCCAGAGTTCGCGTACTACCCGGAACCCAATCCAATC | HindIII | Reverse primer for cloning PvdO-coding region into pEXH5 |
| PfA506-PvdM-DF-MR | ACCGTCGTGGTTGTCGAAGTAG | pvdM genomic deletion control primer | |
| PfA506-PvdM-DR-MR | GGGCCAGCAGTTGGTCCTTGAGTTC | pvdM genomic deletion control primer | |
| PfA506-PvdM-F1-MR | CGCCTTAAGCTTTATGGCGGTGCATGAAGAAGAAGTCG | HindIII | Forward primer for pvdM left flanking region |
| PfA506-PvdM-F2-MR | CCTCAACTGACTGAGATGGCAATGACAAACCGCTAACCACCACGAAGTAAGTCC | Forward primer for pvdM right flanking region | |
| PfA506-PvdM-F-MR | TCAGCGCATATGACAAAATCACGTTCGAAAAAGGCGCTGTATATC | NdeI | Forward primer for cloning PvdM-coding region into pEXH5 |
| PfA506-PvdM-HA-R-MR | GGCTAAAAGCTTTTAGGCGTAGTCCGGCACGTCGTACGGGTAGCGGTTGGCCAAGGGTTTGGCGGCTTTTTGGAC | HindIII | Reverse primer for cloning PvdM-coding region into pEXH5 |
| PfA506-PvdM-R1-MR | TGTCATTGCCATCTCAGTCAGTTGAGG | Reverse primer for pvdM left flanking region | |
| PfA506-PvdM-R2-MR | ATCTGCGGATCCTTGCAGGTGCAACTTGAAGGCTTCG | BamHI | Reverse primer for pvdM right flanking region |
| PfA506-pvdO_D216A-F-MR | CTTTGGCATGTATGCCATGCACGGCAATGT | Primer for D216A exchange by oePCR | |
| PfA506-pvdO_D216A-R-MR | CCGTGCATGGCATACATGCCAAAGGCATTC | Primer for D216A exchange by oePCR | |
| PfA506-PvdO_D257A-F-MR | GCAATGCCTGGGGCGAGGCGCCGGTGTTTT | Primer for D257A exchange by oePCR | |
| PfA506-PvdO_D257A-R-MR | CGCCCCAGGCATTGCCGCGAATCTGGTAGG | Primer for D257A exchange by oePCR | |
| PfA506-PvdO_E179A-F-MR | CCCGTTCGACGCCGGCAAGGGCTACAGCAT | Primer for E179A exchange by oePCR | |
| PfA506-PvdO_E179A-R-MR | CCCTTGCCGGCGTCGAACGGGAAGGGGAAC | Primer for E179A exchange by oePCR | |
| PfA506-pvdO_E260A-F-MR | GCAATGACTGGGGCGCCGCGCCGGTGTTTT | Primer for E260A exchange by oePCR | |
| PfA506-pvdO_E260A-R-MR | GAAAACACCGGCGCGGCGCCCCAGTCATTG | Primer for E260A exchange by oePCR | |
| PfA506-PvdO_H188A-F-MR | ACAGCATCGCCCAGGCCGCCAACACCTACG | Primer for H188A exchange by oePCR | |
| PfA506-PvdO_H188A-R-MR | GTAGGTGTTGGCGGCCTGGGCGATGCTGTA | Primer for H188A exchange by oePCR | |
| PfA506-PvdO_H218A-F-MR | ACCGAATGCCTTTGGCATGTATGACATGGCCGGCAATGTG | Primer for H218A exchange by oePCR | |
| PfA506-PvdO_H218A-R-MR | ATACACATTGCCGGCCATGTCATACATGCCAAAGGCATTC | Primer for H218A exchange by oePCR | |
| PfA506-pvdO_M214A-F-MR | TACCCACCGAATGCCTTTGGCGCCTATGAC | Primer for M214A exchange by oePCR | |
| PfA506-pvdO_M214A-R-MR | GTGCATGTCATAGGCGCCAAAGGCATTCGG | Primer for M214A exchange by oePCR | |
| PfA506-pvdO_M217A-F-MR | CTTTGGCATGTATGACGCCCACGGCAATGT | Primer for M217A exchange by oePCR | |
| PfA506-pvdO_M217A-R-MR | GCCGTGGGCGTCATACATGCCAAAGGCATT | Primer for M217A exchange by oePCR | |
| PfA506-pvdO_N210A-F-MR | AGCTACCCACCGGCCGCCTTTGGCATGTAT | Primer for N210A exchange by oePCR | |
| PfA506-pvdO_N210A-R2-MR | TCATACATGCCAAAGGCGGCCGGTGGGTAG | Primer for N210A exchange by oePCR | |
| PfA506-pvdO_Q187A-F-MR | ACAGCATCGCCGCCCACGCCAACACCTACG | Primer for Q187A exchange by oePCR | |
| PfA506-pvdO_Q187A-R-MR | GTAGGTGTTGGCGTGGGCGGCGATGCTGTA | Primer for Q187A exchange by oePCR | |
| PfA506-pvdO_Y215A-F-MR | TGGCATGGCCGACATGCACGGCAATGTGTA | Primer for Y215A exchange by oePCR | |
| PfA506-pvdO_Y215A-R-MR | GTGCATGTCGGCCATGCCAAAGGCATTCGG | Primer for Y215A exchange by oePCR | |
| PfA506-PvdO-DF-MR | CTGCAGGAAACCTGGAAGCGTGAG | pvdO genomic deletion control primer | |
| PfA506-PvdO-DR-MR | CATGACAAACGAAGCTGTGGCGTGAC | pvdO genomic deletion control primer | |
| PfA506-PvdO-F1-MR | TTAGCCGGATCCCCGAAGGGCTGGCGATGATCTATG | BamHI | Forward primer for pvdO left flanking region |
| PfA506-PvdO-F2-MR | TCCCTTTGAACGAGACGCCTCCATGCGCGAACTCTAATATCTTGTAGG | Forward primer for pvdO right flanking region | |
| PfA506-PvdO-F-MR | TTACCGCATATGACGCCATCCCGACTCAAACCGCTCAC | NdeI | Forward primer for cloning PvdO-coding region into pEXH5 |
| PfA506-PvdO-R1-MR | CATGGAGGCGTCTCGTTCAAAGGG | Reverse primer for pvdO left flanking region | |
| PfA506-PvdO-R2-MR | GCACGCAAGCTTCCTGGTTCGTTCTTGTTCTGTAGG | HindIII | Reverse primer for pvdO right flanking region |
| PfA506-PvdP-DF-MR | GTTGCAGTTCCTTGTCGCTGAGGTTG | pvdP genomic deletion control primer | |
| PfA506-PvdP-DR-MR | CCGGCCAGCATTTCTGGCTATTC | pvdP genomic deletion control primer | |
| PfA506-PvdP-F1-MR | TGCTCTAAGCTTTTGGTCGACATCTGCGACACGTCGATGATCAC | HindIII | Forward primer for pvdP left flanking region |
| PfA506-PvdP-F2-MR | ACGCTTTAAAACGTGTTGCAGGCGCTCTAGG | Forward primer for pvdP right flanking region | |
| PfA506-PvdP-R1-MR | GCGCCTGCAACACGTTTTAAAGCGTTGTCATTGGCTACCTTAGGAAACGGC | Reverse primer for pvdP left flanking region | |
| PfA506-PvdP-R2-MR | TTAAACCCCGGGAGGTACTGGGTAGCCTGTCCAGGCGTATCGACGAG | XmaI | Reverse primer for pvdP right flanking region |