Figure 5.

Naphthol AS-E inhibits IL-10 induction in B cells independently of CREB phosphorylation. A and B, wildtype peritoneal cells were incubated with 20 μm Naphthol AS-E for 1 h. The cells were then either stimulated with 10 μg/ml LPS + brefeldin A + monensin for 5 h or treated with brefeldin A + monensin alone. A and B, intracellular IL-10 in B cells (A) and macrophages (B) was assessed by flow cytometry. Representative plots are shown in the left panels. The data shown in the right panels represent the averages ± standard deviations of three stimulations. ***, p < 0.001 relative to the LPS–stimulated cells (two-tailed unpaired Student's t test). C, as in A except peritoneal cells were isolated from CREB S133A/ATF1 KO mice. D, as in A except peritoneal cells were isolated from MSK1/2 double knockout mice.