Figure 1.

Morphology and pedigree of P3 and newly identified SUN5 mutation. A, morphology of the spermatozoa from ejaculated semen smear, showing that most of the sperm are headless tails, nuclear DNA was stained with DAPI (blue), and acrosomes were stained with FITC-labeled peanut agglutinin (FITC-PNA, green). DIC, differential interference contrast. B, single sperm showing that the decapitated tail contains no DNA and acrosome, whereas the decaudated sperm head contains DNA and acrosome. C, pedigree of the family of P3 with new SUN5 mutation (c.475C→T; p.Arg159*) is shown. The individuals I-1, I-2, and II-1 were Sanger-sequenced. The mutation c.425 + G→A was reported previously.