Figure 2.

Effects of C1 and C2 on actin polymerization with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the fluorescence intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.