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. 2017 Dec 27;293(7):2606–2616. doi: 10.1074/jbc.M117.809137

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Figure 4. — Effect of C2 on endogenous Pfn1–actin interaction in ECs. A, representative fluorescence images of Pfn1–actin PLA spots (arrows) in HmVECs following overnight treatment of either DMSO (control) and C2. An image of the negative control PLA stain (i.e. involving one antibody) only is shown alongside for comparison. Dashed lines indicate the boundaries of the cells (scale bar = 20 μm). B, a dot plot displays the number of PLA spots in C2-treated cells relative to the mean value scored for the DMSO control (the dashed lines represent the mean values (n = 40 cells/group pooled from 2 independent experiments; **, p < 0.01).