Skip to main content
. Author manuscript; available in PMC: 2018 Feb 19.
Published in final edited form as: Angew Chem Int Ed Engl. 2017 Sep 26;56(44):13637–13640. doi: 10.1002/anie.201706696

Figure 3.

Figure 3

In vivo production of poly(2-fluoro-3-hydroxybutyrate-co-3-hydroxybutyrate) by engineered E. coli. (A) Monomer content of polymer and growth medium for full pathway containing the PhaC polymerase (pPOL1, pFMAL2) in the presence of 5 mM fluoromalonate. Data are mean ± SD (n = 3). (B) Expansion of the COSY spectrum of poly(FHB-co-HB). Crosspeak between HB and HC between a fluorinated and non-fluorinated monomer is highlighted in red. (C) Characterization of poly(FHB-co-HB) compared to PHB. (D) 1H-19F HMBC NMR spectrum shows numerous chemically distinct types of FHB monomers (red) as indicated by the number of fluorine crosspeaks between HA, HB, and HC.