Fig. 3. hspb7 mutant zebrafish are subject to exercise-induced mortality.

(A) Kaplan-Meier curve showing survival of hspb7 mutant male and female animals following commencement of breeding at 105 days post fertilization. N = 20 animals (10 male, 10 female). (B) High-intensity exercise paradigm for zebrafish. (C) Representative tracking of fish location in the tank during the final minute of exercise shows that hspb7^WC3/WC3 fish are more likely to remain at the back of the tank. (D) Quantification of fish location in the tank for each second of the final minute of exercise (n = 10). (E) Kaplan-Meier curve showing increase in mortality in hspb7^WC3/WC3 fish following commencement of exercise regime. Dotted lines indicate timing of exercise sessions. For both wildtype and mutant fish n = 20. Significance was calculated using the Graphpad logrank or Mantel-Haenszel test.