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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Immunol Methods. 2017 Dec 16;454:27–31. doi: 10.1016/j.jim.2017.12.001

Figure 2. Evaluation of live CD11c-Gr1-/loMHCII- eosinophils by light microscopy.

Figure 2

A. Light microscopic image of a larger field of eosinophils isolated from lungs of IL5tg mice by the FACS protocol as shown in Fig. 1. Image photographed with a Leica DMI4000 microscope with camera; photographs taken at 40X magnification. B. Percent eosinophils in the initial single cell suspension and in the post-FACS isolate; > 200 cells counted per sample.