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. 2018 Feb 15;12:39. doi: 10.3389/fncel.2018.00039

Figure 2.

Figure 2

Non-degenerating DA neurons in parkin loss-of-function flies do not have fragmented or swollen mitochondria networks. Brains of park mutant flies expressing the mitoGFP construct in TH-producing cells were dissected, stained for TH (blue), and fixed on days 5, 10 or 20 PE. Images in (A) are representative intensity sums of Z-stacks used as 3D projections for the park+/+ and park−/− conditions on day 20. Green (mitoGFP) labeling in the bottom row of (A) represents 3D isosurfaces that are generated based on signal above an automatic bright threshold. Digital image enhancement steps were standardized for green fluorophores. After using standardized image capture parameters, we used Image Pro Premier 3D image analysis software to measure and categorize the mitochondrial object size (B) and inverse of sphericity ([6V]/DS) (C) of mitoGFP objects within the TH-labeled regions for one PPM3 region per brain. Numbers in histogram bars indicate the number of regions analyzed. Error bars represent standard error of the mean; scale bar represents five microns.