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. 2018 Feb 15;9:271. doi: 10.3389/fimmu.2018.00271

Figure 1.

Figure 1

Identification of lung fibrosis in bleomycin (BLM) and phosphate-buffered saline (PBS) groups. Representative histopathological images of lung tissues stained with Masson’s trichrome at early stages (7 days after i.n. instillation of 50 µL of either PBS or 5 mg/kg BLM) (A,B), respectively, and late stages (21 days after i.n. instillation of PBS or BLM) (C,D), respectively. Arrows indicate collagen fibers. (E) Comparison of aniline blue+ collagen areas in lung tissues of different groups. (F) Quantitative expression of mRNA for collagen 1a1 (col1a1), collagen 3a1 (col3a1), and alpha-actin-2 (acta2)/Actb. The letters a, b, c, and d: significant differences between PBS group at 7 days (a), BLM group at 7 days (b), PBS group at 21 days (c), and BLM group at 21 days (d), analyzed by the Kruskal–Wallis test, followed by the Scheffé’s method (P < 0.05); n = 5 in each experimental group. Values are given as the mean ± SE.