Skip to main content
. 2018 Feb 15;9:271. doi: 10.3389/fimmu.2018.00271

Figure 3.

Figure 3

Immune cell populations in mediastinal fat-associated lymphoid clusters (MFALCs) and lungs of bleomycin (BLM)-induced pneumonitis. Densities of T-cells, B-cells, and macrophages in MFALCs (A–C), and their average numbers in lung tissues (D–F). The letters a, b, c, and d: significant differences between phosphate-buffered saline (PBS) group at 7 days (a), BLM group at 7 days (b), PBS group at 21 days (c), and BLM group at 21 days (d), analyzed by Kruskal–Wallis followed by Scheffé’s method (P < 0.05); n = 5 in each experimental group. Values = mean ± SE. The % of T-cells, B-cells, and macrophages in MFALCs were detected by flow cytometry among PBS and BLM groups at 7 days (G) (n ≥ 3 mice of each group). Statistically significant difference, as determined by the Mann–Whitney U-test (p < 0.05); comparison between the BLM and PBS groups at 7 days, with significance indicated by an asterisk (*). Values are shown as the mean ± SE. Flow cytometric analysis of T-cells (CD4/CD8), B-cells (CD19/CD138), and macrophages (M1/M2) subpopulations in MFALCs of PBS group (H), and BLM group (I) at 7 days.