Table 1.

List of antibodies, working dilutions, and methods for antigen retrieval.

Antibody	Source	Dilution	Antigen retrieval	Heating condition
Rabbit anti-CD3	Nichirei (Tokyo, Japan)	1:200	Target retrieval solution (high pH) (produced by Dako, Japan)	105°C, 20 min
Rat anti-B220	Cedarlane (ON, Canada)	1:1,600	10 mM citrate buffer (pH 6.0)	105°C, 20 min
Rabbit anti-Iba1	Wako (Osaka, Japan)	1:1,200	10 mM citrate buffer (pH 6.0)	105°C, 20 min
Rat anti-Gr1	R and D system (MN, USA)	1:800	0.1% pepsin/0.2 N HCl	37°C, 5 min
Rat anti-BrdU	Abcam (Tokyo, Japan)	1:200	10 mM citrate buffer (pH 6.0)	105°C, 20 min
Rat anti-peripheral node addressin	BioLegend (San Diego, CA, USA)	1:500	Target retrieval solution (high pH) (produced by Dako, Japan)	105°C, 20 min
Rabbit anti-LYVE-1	Adipogen (San Diego, CA, USA)	1:500	10 mM citrate buffer (pH 6.0)	105°C, 20 min