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. 2018 Feb 15;9:105. doi: 10.3389/fphar.2018.00105

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Copyright © 2018 Deng, Li, Wu, Wang, Dai, Zhang and Fu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analysis of the surface maker of the FLSs. Mouse IgG isotypes labeled with FITC served as a negative control (A). The positive rate of 0.16% was chosen as the background (as shown in the box) and then the FITC-labeled VCAM-1 (B) expression rate was examined. The results showed that the expression of VCAM-1 in the cell suspension was 99.5%, indicating that the main component of cultured third-generation synoviocytes was FLSs.