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. 2017 Oct 2;6(1):58–71. doi: 10.1002/iid3.191

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© 2017 The Authors. Immunity, Inflammation and Disease Published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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DHS analysis of the Mina locus. (A) Shown is a schematic of the Mina locus (thick horizontal line), DHSs 1–8 (black downward arrowheads), exons 1–10 (black boxes below the thick horizontal line), the Mina transcription unit (horizontal dotted arrow) and 7 and 18.5 kb KpnI (K) fragments (horizontal lines with inward facing arrows; not shown to scale with respect to each other). Activation‐dependent DHS7 and DHS8 are boxed. (B) DHS analysis of EL4 cells activated for 16 h with PMA and ionomycin (PMA/iono) or not (unstim). After nuclei were treated with DNase I (wedges indicate increasing concentration; “‐” indicates no DNaseI control), genomic DNA was digested to completion with KpnI and analyzed by DNA blot with probe C (left) and probe D (right). Constitutive (filled arrowheads) and activation‐dependent (open arrowheads) DHSs are indicated to the right of each panel. Data are representative of two independent experiments with similar results.