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. 2017 Oct 2;6(1):58–71. doi: 10.1002/iid3.191

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© 2017 The Authors. Immunity, Inflammation and Disease Published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Electro‐mobility shift assay of the E2 region spanning SNP17 (rs4191790). (A) Shown is the nucleotide sequence of E2 flanking SNP17 (rs4191790) and a Smad3 binding motif detected by Transfac centered on rs4191790 (rectangle). Horizontal lines depict the extent of overlapping double stranded DNA probes p16‐p20 with green triangles indicating the location of rs4191790. (B) Image of a polyacrylamide gel resolving nucleoprotein complexes formed with rs4191790^G (G) and rs4191790^A (A) allelic versions of p16‐p20 reacted with or without EL4 nuclear extract. Data are representative of two independent experiments.