Figure 4.

Complementary DEC‐gag protein prime rNDV‐L‐gag boost vaccination induces robust balanced CD4⁺ and CD8⁺ T cell immunity. Female CxB6 F1 mice were vaccinated as shown in the y‐axis of Figure 3, 7–10 days after the rNDV‐L‐gag boost, bulk splenocytes were assessed for T cell immunity. Splenocytes were restimulated either with unreactive peptides or with an HIV gagP24 peptide mix, and cytokine production (IFN‐γ, IL‐2, and TNF‐α) in response to peptide was evaluated by intracellular cytokine staining 6 h later in CD4⁺ (a and b) or CD8⁺ (c and d), CD3⁺ T cells. In Figure 3 total IFNγ production is shown for CD4⁺ (a) or CD8+ (c) T cells; whereas in b and c multiple cytokine forming cells are displayed for CD4⁺ and CD8⁺ T cells respectively.