Figure 5.

Complementary DEC‐gag protein prime rNDV‐L‐gag boost vaccination induces robust and durable polyfunctional CD4⁺and CD8⁺ T cell immunity. Female CXB6 F1 mice were vaccinated and T cell immunity measured as described in Figure 4. Cytokine production profile of single cells is assessed as shown in the gating schema in Figure 4a. Seven days after the rNDV‐L‐gag vaccine boost CD4⁺ (b) and CD8⁺ (c) T cells produce multiple cytokines including various combinations of IFN‐γ, IL‐2, and TNF‐α. Fifty days after the rNDV‐L‐gag vaccine boost, we again measured HIV‐1 gag specific cytokine production combinations in single CD4⁺ (d) and CD8⁺ (e) T cells, All data are mean ± SD of 3 experiments involving 5 F1 mice per group.