FIGURE 5.

Pseudonodule formation on the roots of legumes in response to TIBA or NPA treatments. Pseudonodules formed on the roots of (A,B) M. truncatula, (C,D) T. subterraneum (subclover), and (E,F) Sesbania rostrata, in response to treatment with either 50 μM TIBA or 50 μM NPA. (G) Pseudonodules were formed along the entire roots of M. truncatula. The area on the roots encapsulated by the white box indicates the region where the majority of pseudonodules were found on each root. White lines within the box indicate locations of the root tip when the roots were first subjected to TIBA treatment. White arrows indicate pseudonodules found at older parts of the roots. Scale bars represent 1 mm in (A–F) and 1 cm in (G). Abbreviations: TIBA, 2,3,5-triiodobenzoic acid and NPA, 1-N-naphthylphthalamic acid.