Figure 7.

Virus can be found outside the formed callose ring. (A) PVY was detected outside the callose deposits at 6 dpi. (B) High-magnification of the boxed areas in (A) (scale bars are 750 μm). From left to right: chlorophyll fluorescence (red), PVY N605-GFP accumulation (green), callose (blue), overlay of all three channels (scale bars are 2 mm). The infected cell inside the callose ring is shown by the white arrow, while the infected cell outside the callose ring is shown by the yellow arrow.