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. 2018 Feb 19;8:3285. doi: 10.1038/s41598-018-21679-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Anatomical analyses of leaf between wild-type and the SlMED18-RNAi lines. (A and B) The lead of wild type (A) and SMED18-RNAi plant (B) were prepared transverse sections. Bars = 200 μm. (C and D) The estimated cell area (μm²) (C) and cell number of leaf vein (D) were measured and compared between transgenic and wild type at the same position in both cases. Data are shown as the mean ± SE. (E and F) Expression of several genes associated with cell expansion and cell cycle genes (E) SlCycA3;1, SlCycB1;1, SlCycD2;1, SlXTH2; (F) SlPRE1, SlPRE2, SlPRE3, SlPRE4 in leaf.