. 2018 Feb 19;8:3289. doi: 10.1038/s41598-018-21702-5

Table 3.

The different operated conditions of the two PBS/BP reactors (Number 1–12 were sampled from the middle of the two reactors).

		Stage I	Stage II	Stage III	Stage IV	Stage V	Stage VI	Stage VII
Reactor I	Operation conditions and influent characteristics
	Operated time (d)	1–53	54–93	94–120	121–146	147–172	173–190	191–235
	Hydraulic retention time (h)	8	4	2	2	4	1	4
	Salinity (‰)	0	0	0	0	0	0	0
	Influent loading rate (kg ${NO}_{3}^{-}$ -N/m³·d)	0.25 ± 0.02	0.45 ± 0.03	1.01 ± 0.04	0.90 ± 0.09	0.50 ± 0.1	1.94 ± 0.15	0.47 ± 0.05
	${NO}_{3}^{-}$ -N (mg L⁻¹)	100.85 ± 8.07	90.77 ± 5.64	102.47 ± 3.83	91.49 ± 8.98	100.5 ± 19.75	98.40 ± 7.76	95.35 ± 11.03
	${NO}_{2}^{-}$ -N (mg L⁻¹)	0.06 ± 0.07	0.01 ± 0.01	0.08 ± 0.05	0.90 ± 1.84	0.27 ± 0.61	0.03 ± 0.02	0.40 ± 1.39
	TAN (mg L⁻¹)	0.44 ± 0.48	0.14 ± 0.11	0.33 ± 0.18	1.47 ± 1.39	0.12 ± 0.16	0.16 ± 0.09	0.18 ± 0.16
	DOC	71.29 ± 21.16	78.79 ± 13.24	102.77 ± 10.65	90.78 ± 10.81	70.77 ± 12.11	89.8 ± 19.84	90.12 ± 17.94
	DNA sampling
	Sample time (d)	43	93		140	161	187	228
	Number	1	2		3	4	5	6
Reactor II	Operation conditions and influent characteristics
	Operated time (d)	1–53	54–93	94–120	121–146	147–161	162–210	211–235
	Hydraulic retention time (h)	8	4	2	2	4	4	4
	Salinity (‰)	25	25	25	25	25	25	0
	Influent loading rate (kg ${NO}_{3}^{-}$ -N/m³·d)	0.26 ± 0.03	0.46 ± 0.03	1.04 ± 0.06	0.97 ± 0.10	0.50 ± 0.04	0.50 ± 0.04	0.49 ± 0.04
	${NO}_{3}^{-}$ -N (mg L⁻¹)	102.39 ± 7.6	93.83 ± 5.80	105.54 ± 6.55	98.04 ± 9.98	101.16 ± 8.74	101.73 ± 8.83	98.77 ± 8.93
	${NO}_{2}^{-}$ -N (mg L⁻¹)	0.04 ± 0.06	0.00 ± 0.01	0.06 ± 0.03	0.40 ± 0.64	0.23 ± 0.44	0.01 ± 0.03	0.02 ± 0.03
	TAN (mg L⁻¹)	0.42 ± 0.73	0.10 ± 0.10	0.16 ± 0.1	1.51 ± 1.55	0.29 ± 0.65	0.13 ± 0.15	0.10 ± 0.06
	DOC	71.59 ± 24.74	72.09 ± 16.98	100.1 ± 11.86	93.13 ± 12.37	76.7 ± 20.63	108.41 ± 21.3	93.19 ± 10.48
	DNA sampling
	Sample time (d)	43	93		140	161	187	228
	Number	7	8		9	10	11	12