Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 19;8:3305. doi: 10.1038/s41598-018-21642-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

SSE15206 causes apoptosis in drug-resistant cell lines. (A and B) SSE15206 induces PARP cleavage in control and multidrug-resistant cell lines. Both cell line pairs, KB-3–1/KB-V1 and A2780/A2780-Pac-Res were treated with 5× and 10 × GI₅₀ values of SSE15206 or paclitaxel for 24 hours followed by analysis of cleaved-PARP through western blotting. Blots for GAPDH and MDR-1 show loading control and MDR-1 overexpression, respectively in KB-V1 and A2780-Pac-Res cell lines. (C) PARP cleavage and p53 induction by SSE15206 and paclitaxel in HCT116 DMSO and HCT116-Pac-Res cell lines. Cells were treated with 2.5× and 5 × GI₅₀ values of SSE15206 or paclitaxel for 24 hours followed by analysis of PARP cleavage and p53 induction. Levels of GAPDH were used as a loading control. D and E. Effect of 40 μM Verapamil (positive control for inhibition of Pgp) and 10 μM SSE15206 on the efflux of Rhodamine 123 by KB-V1 (D) and A2780-Pac-Res (E) cells. (One-way ANOVA; n.s. = not significant, *p ≤ 0.05). (One-way ANOVA D: F (2, 3) = 19.81, p < 0.05; post-hoc: SSE15206 10 μM vs. DMSO p > 0.05, Verapamil 40 μM vs. DMSO *p < 0.05; One-way ANOVA E: F (2, 3) = 15.99, p < 0.05; post-hoc SSE15206 10 μM vs. DMSO p > 0.05, Verapamil 40 μM vs. DMSO *p < 0.05).