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. 2018 Feb 19;8:3257. doi: 10.1038/s41598-018-21601-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of genistein on mRNA expression of selected differentially expressed genes in MC3T3-E1 cells. (A) Analysis of mRNA on up-regulated selected genes (Ccl7, Lif, Mmp-13, Wisp2, Ereg, Il6, Pde4b, Katnal2, Efcab2, Gdnf) in RNA sequencing. (B) Analysis of mRNA on down-regulated selected genes (Camk2b, Cxcl9, Gli1, Hrc, Ifitm5, Klhl30, Cngb1, Rhbdl2, Susd4) in RNA sequencing. Specific mRNA expression values were normalized to the expression of β-actin. Results are expressed as mean ± S.D of three independent experiments. (*P < 0.05, **P < 0.01 compared with control group).