Table 1.
Top 11 up- and 9 down-regulated genes among 132 differentially expressed genes (q-value < 0.05 and fold change >1.5) in genistein treated mouse osteoblastic cell line MC3T3-E1. Differentially expressed genes were detected b`y RNA-seq analyses.
| Group | Gene Name | Position | log2FC | p-value | q-value | qRT-PCR assay |
|---|---|---|---|---|---|---|
| Up-regulated genes | Katnal2 | 18qE3 | 1.47 | 2.50E-04 | 1.65E-02 | validated |
| Wisp2 | 2qH3 | 1.17 | 5.00E-05 | 4.33E-03 | validated | |
| Mmp13 | 9qA1 | 1.16 | 5.00E-05 | 4.33E-03 | validated | |
| Pde4b | 4qC6 | 1.15 | 5.00E-05 | 4.33E-03 | validated | |
| Ereg | 5qE1 | 1.05 | 5.00E-05 | 4.33E-03 | validated | |
| Efcab2 | 1qH4 | 1.05 | 6.00E-04 | 3.18E-02 | validated | |
| Lif | 11qA1 | 0.88 | 5.00E-05 | 4.33E-03 | validated | |
| Ccl7 | 11qB5 | 0.99 | 5.00E-05 | 4.33E-03 | validated | |
| Ptx3 | 3qE1 | 0.94 | 5.00E-05 | 4.33E-03 | validated | |
| Il6 | 5qB1 | 0.95 | 5.00E-05 | 4.33E-03 | not validated | |
| Gdnf | 15qA1 | 0.92 | 1.50E-04 | 1.07E-02 | not validated | |
| Down-regulated genes | Camk2b | 11qA1 | −1.74 | 5.00E-05 | 4.33E-03 | validated |
| Gli1 | 10qD3 | −1.54 | 4.00E-04 | 2.33E-02 | validated | |
| Hrc | 7qB3 | −1.48 | 5.00E-05 | 4.33E-03 | validated | |
| Ifitm5 | 7qF4 | −1.46 | 5.00E-05 | 4.33E-03 | validated | |
| Klhl30 | 1qD | −1.43 | 5.00E-05 | 4.33E-03 | validated | |
| Col11a2 | 17qB1 | −1.50 | 5.00E-05 | 4.33E-03 | not validated | |
| Susd4 | 1qH5 | −1.39 | 1.50E-04 | 1.07E-02 | not validated | |
| Rhbdl2 | 4qD2.2 | −1.37 | 5.00E-05 | 4.33E-03 | not validated | |
| Cngb1 | 8qC5 | −1.35 | 5.00E-05 | 4.33E-03 | not validated |
FC: fold change.