Figure 10.

Differential effects of Na⁺ channel mutations on the Ca²⁺ dynamics of type Ib, Is, and II synapses. GCaMP Ca²⁺ signals of type Ib and Is (A) and II (B) NMJs from a hypoexcitable allele, para^ts1 (para) and a hyperexcitable allele, para^bss1 (bss) are compared, with representative ΔF/F traces at specified frequencies shown in left panels and the corresponding frequency dependence of max ΔF/F in right panels (mean and SEM, of 6–11 NMJs for each genotype, compare Table 1). Note that type II synaptic boutons in bss frequently displayed “intermittent” or “aborted” GCaMP signals on repetitive stimulation, lowering the average max ΔF/F above 10 Hz (see Fig. 11 for details). KW ANOVA with Bonferroni corrections were performed (*p < 0.05, **p < 0.01).