Figure 11.
Variability in the wave form of GCaMP signals in type II synaptic terminals at high frequencies of stimulation. Representative regular (normal) and irregular (aborted, intermittent, and no response) ΔF/F traces at 20 Hz from WT, eag, eag Sh, bss, and para type II boutons (with c164-GCaMP1.3) are shown. For each genotype, the numbers of NMJs exhibiting each wave form are indicated together with the total sample size (numbers of NMJs examined). N.F., not found. Note that both hypoexcitable (parats1) and hyperexcitable (others) mutations increased the occurrence of irregular waveforms, indicating axonal conduction failure in type II motor terminals (see text). Similar response waveforms were also revealed by GCaMP6m indicator (e.g., for eag1/Y;; nSyb-GCaMP6m/+; normal: 0/6, aborted: 2/6, intermittent: 3/6, no response: 1/6).