Figure 8.

Correlation of giant GCaMP signals with repetitive efEJPs evoked by individual stimuli. A, ΔF/F signals of the GCaMP6m indicator correlated with simultaneous focal recording of efEJPs from the same boutons in type Ib and Is motor terminals (2-Hz stimulation in 0.1 mM Ca²⁺ HL3.1 saline). K⁺ channel blockade with 4-AP (200 µM) and quinidine (Q, 20 µM) in nSyb-GCaMP6m larvae led to giant GCaMP signals in type Is, but not Ib, synapses. Expanded efEJP traces (20 and 100 times, shaded time windows) in B, C indicate supernumerary transmitter releases (arrowheads) following each nerve stimulus (arrow).