Table 1.
Maximum ΔF/F for type I and II synapses of different genotypes
| Genotypes | Type Ib | Type Is | Type II | |||
|---|---|---|---|---|---|---|
| 40 Hz | 2 Hz | 10 Hz | 20 Hz | 2 Hz | 10 Hz | |
| max ΔF/F ± SD (n, N) | max ΔF/F ± SD (n, N) | max ΔF/F ± SD (n, N) | max ΔF/F ± SD (n, N) | max ΔF/F ± SD (n, N) | max ΔF/F ± SD (n, N) | |
| 0.1 mM Ca2+ | ||||||
| WT | 0.16 ± 0.11 (53, 9) | 0.07 ± 0.04 (94, 14) | 0.08 ± 0.07 (96, 14) | 0.43 ± 0.29 (96, 14) | 0.12 ± 0.07 (64, 13) | 0.57 ± 0.39 (67, 13) |
| WT + 4AP | 1.00 ± 0.33 (18, 3)*** | 0.21 ± 0.12 (32, 4)** | 0.83 ± 0.33 (32, 4)*** | 1.14 ± 0.41 (32, 4)*** | 0.24 ± 0.15 (21, 4)** | 1.00 ± 0.40 (21, 4)*** |
| WT + TEA | 0.91 ± 0.43 (22, 4)*** | 0.12 ± 0.07 (32, 5)* | 0.12 ± 0.08 (32, 5)* | 0.86 ± 0.42 (31, 5)*** | 0.71 ± 0.48 (18, 5)*** | 1.02 ± 0.57 (19, 5)*** |
| ShM | 0.63 ± 0.54 (46, 7)*** | 0.11 ± 0.05 (82, 9)* | 0.67 ± 0.27 (82, 9)*** | 1.10 ± 0.40 (81, 9)*** | 0.18 ± 0.07 (44, 6)** | 0.67 ± 0.31 (44, 6) |
| Sh120 | 0.18 ± 0.11 (20, 4) | 0.08 ± 0.05 (42, 4) | 0.63 ± 0.24 (42, 4)*** | 0.99 ± 0.22 (42, 4)*** | 0.18 ± 0.09 (29, 3)* | 0.64 ± 0.23 (29, 3) |
| eag1 | 0.23 ± 0.18 (39, 6)* | 0.09 ± 0.08 (30, 7) | 0.10 ± 0.09 (30, 7) | 0.59 ± 0.32 (28, 7)* | 0.13 ± 0.13 (18, 5) | 0.42 ± 0.37 (20, 5) |
| eag1 Sh120 | 0.59 ± 0.41 (28, 6)*** | 0.43 ± 0.34 (89, 12)*** | 0.83 ± 0.66 (89, 13)*** | 0.89 ± 0.76 (94, 13)*** | 0.17 ± 0.10 (26, 10)* | 0.34 ± 0.33 (26, 9) |
| parabss1 | 0.22 ± 0.21 (49, 6) | 0.07 ± 0.04 (71, 8) | 0.35 ± 0.55 (71, 8)** | 0.64 ± 0.48 (54, 9)** | 0.46 ± 0.26 (56, 10)*** | 0.61 ± 0.39 (55, 10) |
| parats1 | 0.07 ± 0.08 (20, 6)* | 0.08 ± 0.08 (47, 7) | 0.08 ± 0.05 (47, 7) | 0.32 ± 0.20 (47, 7)* | 0.11 ± 0.06 (28, 7) | 0.25 ± 0.20 (28, 7)*** |
| parabss1 Sh120 | 0.36 ± 0.23 (35, 6)*** | 0.17 ± 0.13 (35, 6)** | 0.68 ± 0.46 (35, 6)*** | 0.89 ± 0.64 (35, 6)*** | 0.30 ± 0.24 (22, 5)*** | 0.61 ± 0.40 (22, 5) |
| 0.5 mM Ca2+ | ||||||
| WT | 1.11 ± 0.26 (20, 5)*** | 0.12 ± 0.12 (35, 5)* | 0.43 ± 0.16 (44, 5)*** | 1.22 ± 0.50 (38, 5)*** | 0.33 ± 0.17 (29, 4)*** | 0.85 ± 0.40 (33, 5)** |
| Sh120 | 0.93 ± 0.26 (25, 4)+ | 0.11 ± 0.04 (13, 2) | 0.85 ± 0.24 (22, 3)+++ | 1.15 ± 0.19 (22, 3) | 0.44 ± 0.23 (31, 4) | 1.23 ± 0.52 (31, 4)+++ |
| parabss1 | 0.68 ± 0.14 (26, 3)+++ | 0.08 ± 0.04 (47, 4) | 0.42 ± 0.28 (49, 4) | 1.21 ± 0.55 (48, 4) | 0.73 ± 0.36 (17, 4)+++ | 0.98 ± 0.36 (16, 4) |
Data are presented as max ΔF/F ± SD (n, N), where n indicates total bouton number and N, NMJ number. N.D., not determined. Bolded numbers are significantly different from WT control (0.1 mM Ca2+). Italicized numbers indicate that the ΔF/F traces in the samples (type II) are mostly intermittent. Student’s t tests were performed against WT control of the same frequency in 0.1 mM Ca2+ concentration (*p < 0.05, **p < 0.01, ***p < 0.001) or in 0.5 mM Ca2+ (+p < 0.05, ++p < 0.01, +++p < 0.001).