Table 2.
Primer sequences used in this study.
| Primers | 5′ → 3′ nucleotide sequence | |
|---|---|---|
| efb.fw | ATGGTGAGAATTTATATTTTCAAGGTAGCGAAGGATACGGTCCAAG | |
| efb.rev | TGGGAGGGTGGGATTTTCATTATTTAACTAATCCTTGTTTTAATACATTATC | |
| eap.fw | ATGGTGAGAATTTATATTTTCAAGGTGCAGCTAAGCCATTAGATAAATC | |
| eap.rev | TGGGAGGGTGGGATTTTCATTATTTATTTTTTTTTGATTTAGTGTATTG | |
| sle1.fw | ATGGTGAGAATTTATATTTTCAAGGTGCTACAACTCACACAGTAAAAC | |
| sle1.rev | TGGGAGGGTGGGATTTTCATTAGTGAATATATCTATAATTATTTACTTGGT | |
| sa0710.fw | ATGGTGAGAATTTATATTTTCAAGGTCAACAACATGGCACACAAG | |
| sa0710.rev | TGGGAGGGTGGGATTTTCATTAGTGGATGTAATTATATTTTCCTG | |
| atl(1).fw | ATGGTGAGAATTTATATTTTCAAGGTGCTTCAGCACAACCAAG | |
| atl(1).rev | TGGGAGGGTGGGATTTTCATTATTTTACAGCTGTTTTTGG | |
| atl(2).fw | ATGGTGAGAATTTATATTTTCAAGGTGCTTATACTGTTACTAAACCACAAAC | |
| atl(2).rev | TGGGAGGGTGGGATTTTCATTATTTATATTGTGGGATGTCG | |
| lukG.fw | ATATGGATCCAAGATTAATTCTGAAATCAAACAAGTTTCTG | BamHI |
| lukG.rev | ATATGCGGCCGCTTTCTTTTCATTATCATTAAGTACTTTTAC | NotI |
| lukH.fw | ATATGGATCCGACTCTCAAGACCAAAATAAGAAAG | BamHI |
| lukH.rev | ATATGCGGCCGCTCCTTCTTTATAAGGTTTATTGTCATC | NotI |
| ssaA2.fw | ATATGGATCCTCTGAGCAAGATAACTACGGTTATAATCC | BamHI |
| ssaA2.rev | ATATGCGGCCGCGTGAATGAAGTTATAACCAGCAGCTTGG | NotI |
| emp.fw | ATATGGATCCTCAGTGACAGAGAGTGTTGAC | BamHI |
| emp.rev | ATATGCGGCCGCTACTCGTGGTGCTGGTAAG | NotI |
Underlined are the LIC cloning sequences/restriction sites.