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. 2018 Feb 19;8:3234. doi: 10.1038/s41598-018-21724-z

Table 3.

Identified non covalently cell surface-bound proteins.

Uniprot ID Gene names AA S e Protein name
Proteins with known conserved non covalently cell wall binding domains
LysM (PS51782) a
A6QH29 NWMN_1389 ebpS 486 Elastin-binding protein EbpS
A0A0H3K686 NWMN_0055 spa 520 S Immunoglobulin G binding protein A
A0A0H3K6S5 NWMN_0724 SA0710c 279 S Uncharacterized protein
A0A0H3K7F5 NWMN_0634 265 S Secretory antigen SsaA-like protein
A0A0H3KAZ4 NWMN_0429 sle1d 334 S N-acetylmuramoyl-L-alanine amidase AAA
A0A0H3KG37 NWMN_1157 lytN 383 S Cell-wall hydrolase LytN
GW (PS51780) a
A0A0H3K7X7 NWMN_0922 atl 1256 S Bifunctional autolysin
SH3B (PS51781) a
A0A0H3K875 NWMN_1039 481 Phage amidase [Bacteriophage phiNM2]
A0A0H3K8J7 NWMN_1534 291 S Probable cell wall amidase LytH
G5 (PS51109) a
A0A0H3KAI3 NWMN_2392 1501 S Uncharacterized protein
Identified by surfacome profiling
A0A0H3K7X7 NWMN_0922 atl 1256 S Bifunctional autolysin
A6QIG7 NWMN_1877 chp 149 S Chemotaxis inhibitory protein precursor
A0A0H3KA75 NWMN_0166 coa 636 S Coagulase
A6QF98 NWMN_0758 empd 340 S Extracellular matrix protein-binding protein emp precursor
A0A0H3KF27 NWMN_2399 fnbA 741 S Fibronectin binding protein A
A0A0H3K6R0 NWMN_0687 646 S Lipoteichoic acid synthase
A6QK59 NWMN_2469 isaA 233 S Probable transglycosylase IsaA precursor
A6QJQ7 NWMN_2317 sbi 436 S Immunoglobulin-binding protein sbi precursor
A0A0H3KCA1 NWMN_1066 109 S Uncharacterized protein Ehp
A0A0H3K6X4 NWMN_0362 203 S Uncharacterized protein
A0A0H3KET4 NWMN_0585 168 S Uncharacterized protein
A0A0H3K7N7 NWMN_0757 508 S Secreted von Willebrand factor-binding protein
A0A0H3KEG7 NWMN_2199 ssaA2d 267 S Secretory antigen SsaA
LDS-PAGE: Gel band MS
1b A6QIG2 NWMN_1872 eapd 584 S 65 kDa membrane protein precursor
A6QDC3 NWMN_0083 deoB 392 Phosphopentomutase
2 A6QG68 NWMN_1078 argF 333 Ornithine carbamoyltransferase
3 A6QIG2 NWMN_1872 eapd 584 S 65 kDa membrane protein precursor
4 A6QJQ7 NWMN_2317 sbi 436 S Immunoglobulin-binding protein Sbi precursor
5 A0A0H3KHT5 NWMN_1928 lukHd 351 S Leukocidin/hemolysin toxin family S subunit
6A6QF98 NWMN_0758 empd 340 S Extracellular matrix protein-binding protein Emp precursor
  A0A0H3K9N1 NWMN_1927 lukGd 338 S Leukocidin/hemolysin toxin family F subunit
7A0A0H3K5Z1 NWMN_0249 held 296 S 5′-nucleotidase, lipoprotein e(P4) family protein
  A0A0H3KIY0 NWMN_2444 85 Uncharacterized protein
8A6QG59 NWMN_1069 efbd 165 S Fibrinogen-binding protein precursor

aPROSITE ID of Motif; bExtracted band number from LDS-PAGE; cGene locus of NWMN_0724 homolog in S. aureus N315, used instead of gene name; dGene name of homologous protein if none was found in the Uniprot record; Se Secretion signal predicted by SignalP and/or according to Uniprot.