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NLP assembly methods. (A) Scaffold protein of interest, lipids, and detergent are mixed, and detergent is removed to allow NLPs to form. (B) Plasmid encoding the scaffold protein of interest is mixed with lipids (and telodendrimers, for telodendrimer‐NLP discs) in a cell‐free reaction chamber. In both (A) and (B), correctly formed NLPs/telodendrimer‐NLPs are purified using SEC.
