Figure 3.

Stabilization of split intein fusions through extein extension. (A) Schematic depicting the extein extension strategy applied to stabilize a fragmented extein (gray). (B) Coomassie‐stained SDS‐PAGE gel of the Cfa N‐intein fused to full length SUMO (Sumo‐Cfa^N), truncated SUMO (Sumo_trunc‐Cfa^N), or extended SUMO (Sumo_ext‐Cfa^N). Lanes corresponding to whole cell lysate (W), inclusion body pellet (P), soluble lysate (S), flow through (FT), and elution following Ni‐NTA enrichment (E) are shown. (C) Quantification of normalized protein signal from the Ni‐NTA enrichment lane highlighted in panel b (red box). Error = SD (n = 3).