Figure 1.

Construction of positional scanning‐synthetic combinatorial libraries for analysis of prime side specificity. (A) During solid‐phase peptide synthesis, 7‐amino‐4‐methylcoumarin (AMC)‐based positional scanning‐synthetic combinatorial libraries are generally conjugated to the solid support via the side chain of the P1 amino acid, while 7‐amino‐4‐carbamoylmethylcoumarin (ACC)‐based libraries are conjugated directly through the fluorophore. (B) The four P_n sublibraries each contain 20 distinct pools of substrates, where one amino acid is fixed at P_n position and the remaining positions (X) contain an equimolar mixture of amino acids.