Figure 1.

ISL1 is upregulated in breast cancer, and the expression of ISL1 is corrected with tumor size and metastasis.

Notes: (A) The mRNA level of ISL1 in MDA-MB-231 and MCF-7 cells was determined by qRT-PCR; MCF-10A was used as the control group. *p<0.05, MDA-MB-231 or MCF-7 vs MCF-10A. (B) The protein level of ISL1 in MDA-MB-231 and MCF-7 cells was determined by Western blotting; MCF-10A was used as the control group. (C) The protein level of ISL1 in breast cancer tissue samples was determined by Western blotting; adjacent normal tissue samples were used as the control group. *p<0.05, tumor tissues vs normal tissues. (D) The mRNA level of ISL1 in breast cancer tissue samples was determined by qRT-PCR; adjacent normal tissue samples were used as the control group. (E) The expression of ISL1 in breast cancer was assessed on a website (http://www.cbioportal.org). MBL (sickkids 2016): Mutational profiles of metastatic breast cancer (France, 2016). (F) The survival curve in 108 breast cancer patients according to ISL1 expression was analyzed by the Kaplan–Meier method.

Abbreviations: Cum, cumulative; qRT-PCR, quantitative real-time polymerase chain reaction.