Figure 2.

ISL1 facilitates proliferation of breast cancer cells.

Notes: (A) MCF-7 cells were treated with vector, ISL1 or SCR, ISL1 siRNA (siISL1), respectively. The mRNA level of ISL1 was detected by qRT-PCR. (B) ISL1 was overexpressed or knocked down in MCF-7 cells; the protein level of ISL1 was detected by Western blotting. (C) ISL1 was overexpressed or knocked down in MCF-7 cells; the colony formation analysis was performed to detect the effect of ISL1 on cell proliferation. The graph indicated the relative number of colonies. (D) ISL1 was overexpressed or knocked down in MCF-7 cells; the CCK-8 assay was performed to assess the proliferation ability of MCF-7 affected by ISL1. *p<0.05, ISL1 vs vector, siISL1 vs SCR.

Abbreviations: OD, optical density; qRT-PCR, quantitative real-time polymerase chain reaction; SCR, scramble siRNA.