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Flow chart showing the adopted methods for UTI purification (anion exchange chromatography) and analysis of both protein core (SDS-PAGE followed by MS analysis) and chondroitin sulphate moiety (fluorophore-assisted carbohydrate electrophoresis analysis). Cellulose acetate electrophoresis was performed to assess effectiveness of UTI purification. I elution: UTI and UTI derivatives; II elution: heparan sulphate (HS) and free highly charged chondroitin sulphate (CS).
