Figure 7.

Effect of citrate transporter inhibitors on ΔΨm and activity of CPT-1 in HepG2 cells. (a) The loss of ΔΨm was determined by JC-1 staining and detected by flow cytometry. Cells were treated with 10 μg/ml of TOFA for 24 h, pretreated with 10 μg/ml of TOFA for 1 h followed by treatment with CTPi, PMCTi, and combined CTPi and PMCTi for 24 h. (b) Percentage of CPT-1 activity was determined in cells treated with each inhibitor and combined inhibitors and 0.1 mM of C75 for 24 h. The control was defined as a vehicle for which cells were treated with a medium containing 0.2% DMSO alone. Data are presented as mean ± SEM of three independent experiments, ^∗ρ < 0.05.