Table 2.

Four pairs of primers were used to amplify and synthesize the human BMPR-IA gene for the following mutations (MT -349C > T, MT 4A > C) by PCR using pcDNA3.1/BMPR-IA wild type plasmid as the template

SNPs	Primer	Forward	Reverse	Annealing Temperature (°C)
-349C > T	Primer 1	CCGGAATTCACCATGGTGGCCGGGACC	CCGACGCCGCCGCCGCGAACTT	60
	Primer 2	TCGCGGCGGCGGCGTCGG	CCGCTCGAGCTAGCGACACCCACAACCCTCCA	60
4A > C	Primer 1	CCGGAATTCACCATGGTGGCCGGGACC	CTGGTGTCCAAAAGTCTGGTCACGGG	60
	Primer 2	CCCCGTGACCAGACTTTTGGACACC	CCGCTCGAGCTAGCGACACCCACAACCCTCCA	60